Professor, Head of Proteomics Department,
Coordinator of Monoclonal Antibody Laboratory
Institute of Cellular Biology and Pathology N. Simionescu, Romanian Academy, Bucharest
Felicia Antohe’s research activities cover cell receptors and traffic in endothelial cells in normal and pathological conditions, bio-pathology of atherosclerosis, diabetes, and immune disorders, etc. She and her research group have important results on transport of macromolecules in vascular cells: transcytosis, endocytosis, on monoclonal antibodies as diagnostic and therapeutic tools, on proteomic of endothelial cells in health and disease, on structural and functional characterization of micro-domains, lipid rafts and caveolae and on inflammatory systemic diseases.
She and her group use in their research immunological methods (monoclonal antibodies, FACS, ELISA), cell Biology methods (cell culture, animal models, microsurgery, electron microscopy, immunocytochemistry, fluorescence microscopy, protein labeling, western blotting, DNA and protein electrophoresis, gel and affinity chromatography), vascular cell proteomics and Radiobiochemistry methods (1D and 2D SDS-PAGE, protein radio-labeling, scintillation and gamma counting, autoradiography at LM and EM level) and molecular biology methods (PCR, RT-qPCR, in situ hybridization, plasmid transfection).
She was the manager/coordinator of more than 44 research projects gained by competition from national and international programs and is the author/co-author of more than 170 peered reviewed articles.